Volume 81, no. 4, p. 1934-1950, 2007: Page 1936, Materials and Methods, Antibodies, lines 4 and 5: The source for the anti-ATM rabbit polyclonal and anti-pATM (Ser 1981) mouse monoclonal (clone 10H11.E12) antibody was Novus Biologicals, not BD Bioscience.
The sources for Rad51 and Actin Abs were inadvertently omitted, and the following sentence should be added at the end of the paragraph. “Rad51 mouse monoclonal Abs were either from Labvision (clone 3C10 used for Western blots) or from Abcam (clone 14B4 used for IF). The mouse monoclonal actin Ab (pan Ab-5 clone C4) was from Thermo Scientific.”
Page 1937, Fig. 1D: The pATM level at 4 h p.i. in the viral sample showed a very minor change at this time point, which we interpreted as essentially no change. We have since repeated this very early time course several more times, with further refinement between 3 to 7 h p.i., mapping at half-hour intervals. In these experiments, we have consistently observed this very minor and transient increase in pATM levels occurring sometime between 3.5 and 5.5 h p.i. This very minor change coincides with the increases in pNBS1 and pp53 levels observed during this time period and may explain the initiation of phosphorylation of these two proteins during infection. This supports our conclusion that ATM was responsible for these very early phosphorylation events, as demonstrated by the lack of phosphorylation of NBS1 and p53 at early times in ATM-minus cell lines.
Page 1944, Fig. 7A: The image displayed for Rad51 localization in virus-infected cells at 48 h p.i. was incorrect. Rad51 is strongly sequestered into the viral replication centers at this time point and should have been classified as being in Group 1, not in Group 3. Below is a corrected image:
Figure 1.
Page 1945, Discussion: We regret that the reference for Castillo et al. (11a) was inadvertently omitted from our discussion of the phosphorylation of p53 and ATM during HCMV infection.
Page 1949: The following reference was inadvertently omitted.
11a. Castillo, J. P., F. M. Frame, H. A. Rogoff, M. T. Pickering, A. D. Yurochko, and T. F. Kowalik. 2005. Human cytomegalovirus IE1-72 activates ataxia telangiectasia mutated kinase and a p53/p21-mediated growth arrest response. J. Virol. 79: 11467-11475.
None of these minor changes in any way affects the conclusions of our published study.
We thank Amit Kulkarni for his efforts in addressing these minor corrections to our study.