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. 2001 Mar 6;98(6):3489–3494. doi: 10.1073/pnas.061028798

Figure 2.

Figure 2

(A–C) Relative densities of β2/3 subunit-containing clusters with presynaptic contacts. Synaptically located receptors increase with increasing age of the cultures. However, the majority of all β2/3 clusters are lacking presynaptic partners even in well-differentiated cultures. Colocalizations with FM4-64 or SV2-positive synapses are maximal at day 30 (A and B). The highest density of synaptic β2/3 clusters at GABAergic terminals was measured on day 20 (C). *, P < 0.05; ***, P < 0.001 vs. day 10. †, P < 0.05 vs. day 30. (D–F) Relative densities of γ2 subunit-containing clusters with presynaptic contacts. Synaptic receptor clusters containing γ2 increase with increasing age of the cultures, although the majority of the clusters remain without a presynaptic partner, even in mature cultures on day 30. With GAD-stained synapses, the density is highest on day 20 (F). ***, P < 0.0001 vs. day 5; †, P < 0.002 vs. day 10 and P < 0.05 vs. day 20; **, P < 0.02 vs. day 10; §, P < 0.0001 vs. days 5 and 10.