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. 1995 Apr 11;23(7):1165–1169. doi: 10.1093/nar/23.7.1165

Molecular cloning of a small DNA binding protein with specificity for a tissue-specific negative element within the rps1 promoter.

D X Zhou 1, C Bisanz-Seyer 1, R Mache 1
PMCID: PMC306826  PMID: 7739894

Abstract

A cDNA encoding a specific binding activity for the tissue-specific negative cis-element S1F binding site of spinach rps1 was isolated from a spinach cDNA expression library. This cDNA of 0.7 kb encodes an unusual small peptide of only 70 amino acids, with a basic domain which contains a nuclear localization signal and a putative DNA binding helix. This protein, named S1Fa, is highly conserved between dicotyledonous and monocotyledonous plants and may represent a novel class of DNA binding proteins. The corresponding mRNA is accumulated more in roots and in etiolated seedlings than in green leaves. This expression pattern is correlated with the tissue-specific function of the S1F binding site which represses the rps1 promoter preferentially in roots and in etiolated plants.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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