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. 1995 Apr 11;23(7):1170–1176. doi: 10.1093/nar/23.7.1170

Looped oligonucleotides form stable hybrid complexes with a single-stranded DNA.

E Azhayeva 1, A Azhayev 1, A Guzaev 1, J Hovinen 1, H Lönnberg 1
PMCID: PMC306827  PMID: 7739895

Abstract

Several new branched (1, 2), circular (9) and looped oligonucleotides (14-17) were synthesized. 3'-Deoxypsicothymidine was employed to create the site of branching when required. The circular and looped structures were obtained by oxidative disulfide bond formation between mercaptoalkyl tether groups. All the oligonucleotides prepared contained two T11 sequences, and the branched and looped oligomers an additional alternating CT sequence. The melting experiments revealed that the branched oligonucleotides form relatively weak hybrid (double/triple helix) complexes with the single-stranded oligodeoxyribonucleotide, showing a considerable destabilizing effect produced by the structure at the point of branching. The data obtained with looped oligonucleotides demonstrated considerable stabilization of the hybrid (double/triple helix) complexes with the complement. The data reported may be useful in attempting to design new antisense or antigene oligonucleotides capable of forming selective and stable bimolecular hybrid complexes with nucleic acids.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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