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. 1995 May 25;23(10):1834–1835. doi: 10.1093/nar/23.10.1834

Combinatorial libraries by cassette mutagenesis.

B Borrego 1, A Wienecke 1, A Schwienhorst 1
PMCID: PMC306945  PMID: 7784192

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Crouse G. F., Frischauf A., Lehrach H. An integrated and simplified approach to cloning into plasmids and single-stranded phages. Methods Enzymol. 1983;101:78–89. doi: 10.1016/0076-6879(83)01006-x. [DOI] [PubMed] [Google Scholar]
  2. Dube D. K., Loeb L. A. Mutants generated by the insertion of random oligonucleotides into the active site of the beta-lactamase gene. Biochemistry. 1989 Jul 11;28(14):5703–5707. doi: 10.1021/bi00440a001. [DOI] [PubMed] [Google Scholar]
  3. Dugaiczyk A., Boyer H. W., Goodman H. M. Ligation of EcoRI endonuclease-generated DNA fragments into linear and circular structures. J Mol Biol. 1975 Jul 25;96(1):171–184. doi: 10.1016/0022-2836(75)90189-8. [DOI] [PubMed] [Google Scholar]
  4. Dunn I. S., Cowan R., Jennings P. A. Improved peptide function from random mutagenesis over short 'windows'. Protein Eng. 1988 Oct;2(4):283–291. doi: 10.1093/protein/2.4.283. [DOI] [PubMed] [Google Scholar]
  5. Hall L., Emery D. C. A rapid and efficient method for site-directed mutagenesis by PCR, using biotinylated universal primers and streptavidin-coated magnetic beads. Protein Eng. 1991 Jun;4(5):601–601. doi: 10.1093/protein/4.5.601. [DOI] [PubMed] [Google Scholar]
  6. Hermes J. D., Blacklow S. C., Knowles J. R. Searching sequence space by definably random mutagenesis: improving the catalytic potency of an enzyme. Proc Natl Acad Sci U S A. 1990 Jan;87(2):696–700. doi: 10.1073/pnas.87.2.696. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Higuchi R., Krummel B., Saiki R. K. A general method of in vitro preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions. Nucleic Acids Res. 1988 Aug 11;16(15):7351–7367. doi: 10.1093/nar/16.15.7351. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ho S. N., Hunt H. D., Horton R. M., Pullen J. K., Pease L. R. Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene. 1989 Apr 15;77(1):51–59. doi: 10.1016/0378-1119(89)90358-2. [DOI] [PubMed] [Google Scholar]
  9. Horwitz M. S., Loeb L. A. DNA sequences of random origin as probes of Escherichia coli promoter architecture. J Biol Chem. 1988 Oct 15;263(29):14724–14731. [PubMed] [Google Scholar]
  10. Horwitz M. S., Loeb L. A. Promoters selected from random DNA sequences. Proc Natl Acad Sci U S A. 1986 Oct;83(19):7405–7409. doi: 10.1073/pnas.83.19.7405. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kaiser C. A., Preuss D., Grisafi P., Botstein D. Many random sequences functionally replace the secretion signal sequence of yeast invertase. Science. 1987 Jan 16;235(4786):312–317. doi: 10.1126/science.3541205. [DOI] [PubMed] [Google Scholar]
  12. Munir K. M., French D. C., Dube D. K., Loeb L. A. Permissible amino acid substitutions within the putative nucleoside binding site of herpes simplex virus type 1 encoded thymidine kinase established by random sequence mutagenesis [corrected]. J Biol Chem. 1992 Apr 5;267(10):6584–6589. [PubMed] [Google Scholar]
  13. Oliphant A. R., Struhl K. An efficient method for generating proteins with altered enzymatic properties: application to beta-lactamase. Proc Natl Acad Sci U S A. 1989 Dec;86(23):9094–9098. doi: 10.1073/pnas.86.23.9094. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Reidhaar-Olson J. F., Sauer R. T. Combinatorial cassette mutagenesis as a probe of the informational content of protein sequences. Science. 1988 Jul 1;241(4861):53–57. doi: 10.1126/science.3388019. [DOI] [PubMed] [Google Scholar]
  15. Zaug A. J., Been M. D., Cech T. R. The Tetrahymena ribozyme acts like an RNA restriction endonuclease. Nature. 1986 Dec 4;324(6096):429–433. doi: 10.1038/324429a0. [DOI] [PubMed] [Google Scholar]
  16. Zoller M. J., Smith M. Oligonucleotide-directed mutagenesis using M13-derived vectors: an efficient and general procedure for the production of point mutations in any fragment of DNA. Nucleic Acids Res. 1982 Oct 25;10(20):6487–6500. doi: 10.1093/nar/10.20.6487. [DOI] [PMC free article] [PubMed] [Google Scholar]

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