Figure 2.—

Tetrad analysis of knockout strains. Asci from the appropriate new∷nat/new⁺ ade6.M210/ade6.M216 h⁺/h− diploid strains were dissected on rich yeast extract supplemented (YES) medium at 25°. Images of colony formation on the left demonstrate the essentiality of new21⁺ and the slow-growth phenotypes of new1∷nat, tam7∷nat, and new8∷nat cells. The bright-field microscopy images show increased magnification of new/tam⁺ (middle) and newx∷nat/tamx∷nat (right) colonies (denoted as “Wild type” and “Deletion”). The far right panel of the new8∷nat cells shows the inability of the microcolonies formed at 25° to grow 4 days after restreaking onto fresh YES at 25°.