Abstract
Avian myeloblastosis virus and Maloney murine leukemia virus RNase H-reverse transcriptases pause when they encounter a 2'-5' linkage or a 2'-thiophosphate in their template RNAs, but eventually read through these backbone modifications. Both reverse transcriptases pause after the 2'-5' linkage but before the 2'-thiophosphate. These results suggest that in the absence of precise information concerning the behavior of a given reverse transcriptase with respect to a particular lesion or modification, caution should be exercised in the interpretation of primer extension data that is being used to determine the existence of, or map the position of, a crosslink, site of chemical modification or non-standard linkage in an RNA template.
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- Bakhanashvili M., Hizi A. Fidelity of the RNA-dependent DNA synthesis exhibited by the reverse transcriptases of human immunodeficiency virus types 1 and 2 and of murine leukemia virus: mispair extension frequencies. Biochemistry. 1992 Oct 6;31(39):9393–9398. doi: 10.1021/bi00154a010. [DOI] [PubMed] [Google Scholar]
- Bartel D. P., Szostak J. W. Isolation of new ribozymes from a large pool of random sequences [see comment]. Science. 1993 Sep 10;261(5127):1411–1418. doi: 10.1126/science.7690155. [DOI] [PubMed] [Google Scholar]
- Denman R., Colgan J., Nurse K., Ofengand J. Crosslinking of the anticodon of P site bound tRNA to C-1400 of E.coli 16S RNA does not require the participation of the 50S subunit. Nucleic Acids Res. 1988 Jan 11;16(1):165–178. doi: 10.1093/nar/16.1.165. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Domdey H., Apostol B., Lin R. J., Newman A., Brody E., Abelson J. Lariat structures are in vivo intermediates in yeast pre-mRNA splicing. Cell. 1984 Dec;39(3 Pt 2):611–621. doi: 10.1016/0092-8674(84)90468-9. [DOI] [PubMed] [Google Scholar]
- Lorsch J. R., Szostak J. W. In vitro evolution of new ribozymes with polynucleotide kinase activity. Nature. 1994 Sep 1;371(6492):31–36. doi: 10.1038/371031a0. [DOI] [PubMed] [Google Scholar]
- Mendelman L. V., Petruska J., Goodman M. F. Base mispair extension kinetics. Comparison of DNA polymerase alpha and reverse transcriptase. J Biol Chem. 1990 Feb 5;265(4):2338–2346. [PubMed] [Google Scholar]
- Mörl M., Niemer I., Schmelzer C. New reactions catalyzed by a group II intron ribozyme with RNA and DNA substrates. Cell. 1992 Sep 4;70(5):803–810. doi: 10.1016/0092-8674(92)90313-2. [DOI] [PubMed] [Google Scholar]
- Rodriguez J. R., Pikielny C. W., Rosbash M. In vivo characterization of yeast mRNA processing intermediates. Cell. 1984 Dec;39(3 Pt 2):603–610. doi: 10.1016/0092-8674(84)90467-7. [DOI] [PubMed] [Google Scholar]
- Youvan D. C., Hearst J. E. Reverse transcriptase pauses at N2-methylguanine during in vitro transcription of Escherichia coli 16S ribosomal RNA. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3751–3754. doi: 10.1073/pnas.76.8.3751. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zeitlin S., Efstratiadis A. In vivo splicing products of the rabbit beta-globin pre-mRNA. Cell. 1984 Dec;39(3 Pt 2):589–602. doi: 10.1016/0092-8674(84)90466-5. [DOI] [PubMed] [Google Scholar]