Table 1.

Methods used by participating laboratories for detection of XMRV nucleic acids in whole blood samples

Lab	Extraction method	Blood Volume (µl)	Input DNA (ng)	Assay #	Target	Assay type	Quant/ nested	Primer Reference
CDC	QIAamp mini blood	400	1000	1	gag	PCR southern	Nested	9
				2	pol	PCR southern	Nested	9
FDA (H)a	QIAamp mini blood	200	500–1000		gag	PCR	Nested	2
FDA (Lo)	Qiagen DNeasy blood	200	30–50	1	gag	PCR	Nested	4
			30–50	2	gag	PCR	Nested	4
GPb	Magnetic-based target capture	50	N/A	Duplex	Proprietaryc	TMAc	N/A	Unpublished
NCI	Promega Wizard Genomic DNA	500	4000		5’ UTR of gag	PCR SCAd	Quant	Unpublishedd
WPI	QIAamp DNA mini blood	250	100		5’ UTR of gag	qPCR	Quant	24

^aH=Hewlett

^bGP=Gen-Probe Inc

^cGen-Probe employs a duplex transcription-mediated amplification (TMA) assay based on multiple proprietary probes

^dSCA=single-copy assay.

Forward primer: 5-TGTATCAGTTAACCTACCCGAGT-3’; Reverse primer: 5-AGACGGGGGCGGGAAG-3’; XMRV probe 5’fam-TGGAGTGGCTTTGTTGGGGGACGA- tamra3’