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Phase contrast (column 1) and H&E-stained (columns 2 &3) cell images show changes in primary fibroblast morphology over time. Columns 1 and 2 are at a 40× magnification and column 3 is at a 10× magnification. Day 1 cells possessed spindle and stellate morphologies. By Day 15, cells began to develop larger cell bodies and nuclei. By day 30, the cells possessed very large cell bodies, with some containing multiple and pleomorphic nuclei.
