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. 1995 Sep 25;23(18):3638–3641. doi: 10.1093/nar/23.18.3638

Identification of the factors that interact with NCBP, an 80 kDa nuclear cap binding protein.

N Kataoka 1, M Ohno 1, I Moda 1, Y Shimura 1
PMCID: PMC307259  PMID: 7478990

Abstract

It has been shown that the monomethylated cap structure plays important roles in pre-mRNA splicing and nuclear export of RNA. As a candidate for the factor involved in these nuclear events we have previously purified an 80 kDa nuclear cap binding protein (NCBP) from a HeLa cell nuclear extract and isolated its full-length cDNA. In this report, in order to obtain a clue to the cellular functions of NCBP, we attempted to identify a factor(s) that interacts with NCBP. Using the yeast two-hybrid system we isolated three clones from a HeLa cell cDNA library. We designated the proteins encoded by these clones NIPs (NCBP interacting proteins). NIP1 and NIP2 have an RNP consensus-type RNA binding domain, whereas NIP3 contains a unique domain of Arg-Glu or Lys-Glu dipeptide repeats. We also show that NCBP requires NIP1 for binding to the cap structure. Possible roles of NIPs in cap-dependent nuclear processes are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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