Table 1.
Oligonucleotide primers used in this study
| Primer designation | Nucleotide sequence (5′ → 3′)a |
| P1F | TTTGAACGTGCGATGAAAG |
| P2R | GATTGTGGAACTGATGTAACAG |
| P3F | GCGGATCCATTGAAGAAACTGCTGAAGCTC |
| P4R | GCGGATCCAGGAATAGATGTATATCAGAAC |
| P5F | CGTTAAAGCCTGATGTAAGCA |
| P6R | GGTTACGTGGTTGCTTAC |
| P9F | GCGGATCCTTAAAGTATTATAGCACGACTG |
| P10R | GCTCTACAATATCCAGTGTCGAAACGTC |
| P11F | GCGGTACCTTTGAACGTGCGATGAAAG |
| P12R | GCGGTACCGATTGTGGAACTGATGTAACAG |
| BAluxSF | ATGCATCAGTAGAAAGCTTTG |
| BAluxSR | TTATCCAAATACTTTCTCAAGTTC |
a
Restriction sites underlined; BamHI (GGATCC) and KpnI (GGTACC).