Skip to main content
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1995 Oct 11;23(19):4003–4004. doi: 10.1093/nar/23.19.4003

A lacZ-hygromycin fusion gene and its use in a gene trap vector for marking embryonic stem cells.

D Natarajan 1, C A Boulter 1
PMCID: PMC307326  PMID: 7479052

Abstract

Full text

PDF
4003

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boulter C. A., Aguzzi A., Williams R. L., Wagner E. F., Evans M. J., Beddington R. Expression of v-src induces aberrant development and twinning in chimaeric mice. Development. 1991 Feb;111(2):357–366. doi: 10.1242/dev.111.2.357. [DOI] [PubMed] [Google Scholar]
  2. Friedrich G., Soriano P. Promoter traps in embryonic stem cells: a genetic screen to identify and mutate developmental genes in mice. Genes Dev. 1991 Sep;5(9):1513–1523. doi: 10.1101/gad.5.9.1513. [DOI] [PubMed] [Google Scholar]
  3. Gossler A., Joyner A. L., Rossant J., Skarnes W. C. Mouse embryonic stem cells and reporter constructs to detect developmentally regulated genes. Science. 1989 Apr 28;244(4903):463–465. doi: 10.1126/science.2497519. [DOI] [PubMed] [Google Scholar]
  4. Gritz L., Davies J. Plasmid-encoded hygromycin B resistance: the sequence of hygromycin B phosphotransferase gene and its expression in Escherichia coli and Saccharomyces cerevisiae. Gene. 1983 Nov;25(2-3):179–188. doi: 10.1016/0378-1119(83)90223-8. [DOI] [PubMed] [Google Scholar]
  5. Kerr W. G., Nolan G. P., Serafini A. T., Herzenberg L. A. Transcriptionally defective retroviruses containing lacZ for the in situ detection of endogenous genes and developmentally regulated chromatin. Cold Spring Harb Symp Quant Biol. 1989;54(Pt 2):767–776. doi: 10.1101/sqb.1989.054.01.090. [DOI] [PubMed] [Google Scholar]
  6. Lupton S. D., Brunton L. L., Kalberg V. A., Overell R. W. Dominant positive and negative selection using a hygromycin phosphotransferase-thymidine kinase fusion gene. Mol Cell Biol. 1991 Jun;11(6):3374–3378. doi: 10.1128/mcb.11.6.3374. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Skarnes W. C., Auerbach B. A., Joyner A. L. A gene trap approach in mouse embryonic stem cells: the lacZ reported is activated by splicing, reflects endogenous gene expression, and is mutagenic in mice. Genes Dev. 1992 Jun;6(6):903–918. doi: 10.1101/gad.6.6.903. [DOI] [PubMed] [Google Scholar]
  8. Wilson V., Manson L., Skarnes W. C., Beddington R. S. The T gene is necessary for normal mesodermal morphogenetic cell movements during gastrulation. Development. 1995 Mar;121(3):877–886. doi: 10.1242/dev.121.3.877. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES