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Deletion of ExsC does not reduce the synthesis of ExsA under inducing conditions. For these experiments exsA (chromosomal) was tagged with the HA antigen sequence in both the NY-4 (lanes 1 and 2) and ΔexsC (lanes 3 and 4) strains. After growth in LB-S (lanes 1 and 3) for 4 h or with HeLa cells (lanes 2 and 4) for 4 h, whole-cell lysates were collected and probed with anti-HA (upper) or anti-DnaK (lower) by western blot analysis.
