Figure 3.

Preparation of antifungal-drug containing food vials and fly fasting. (A) A spatula is used to create 2- to 3-mm-deep abrasions on the surface of the fly food. Afterward, 200 µl of the antifungal drug of choice is added to the surface. (B) Dry yeast particles are then added to the surface of the drug-containing vial until they are entirely soaked by the antifungal agent. (C) Before exposure to the drug-containing food vials, flies are placed in empty vials for 6–8 hours so that they can starve.