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. 1995 Oct 25;23(20):4050–4054. doi: 10.1093/nar/23.20.4050

Cell cycle-dependent regulation of RNA polymerase II basal transcription activity.

M Yonaha 1, T Chibazakura 1, S Kitajima 1, Y Yasukochi 1
PMCID: PMC307341  PMID: 7479063

Abstract

Regulation of transcription by RNA polymerase II (pol II) in eukaryotic cells requires both basal and regulatory transcription factors. In this report we have investigated in vitro pol II basal transcription activity during the cell cycle by using nuclear extracts from synchronized HeLa cells. It is shown that pol II basal transcription activity is low in the S and G2 phases and high in early G1 phase and TFIID is the rate limiting component of pol II basal transcription activity during the cell cycle. Further analyses reveal that TFIID exists as a less active form in the S and G2 phases and nuclear extracts from S and G2 phase cells contain a heat-sensitive repressor(s) of TATA box binding protein (TBP). These results suggest that pol II basal transcription activity is regulated by a qualitative change in the TFIID complex, which could involve repression of TBP, during the cell cycle.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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