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. 1995 Oct 25;23(20):4127–4133. doi: 10.1093/nar/23.20.4127

Isolation of YAC insert sequences by representational difference analysis.

M Schutte 1, L T da Costa 1, C A Moskaluk 1, E Rozenblum 1, X Guan 1, P J de Jong 1, M Bittner 1, P S Meltzer 1, J M Trent 1, S E Kern 1
PMCID: PMC307354  PMID: 7479076

Abstract

We present a method for the isolation of YAC insert sequences by representational difference analysis (RDA). To achieve maximal representation of the sequences, the amplicons were generated from a Mbol digestion product. RDA was performed using a 970 kb insert YAC clone. After two rounds of re-association and selective amplification 92% of the difference product represented sequences derived from the YAC insert. Twenty insert-specific sequence-tagged sites were readily defined. The difference product was also successfully used to isolate microsatellite markers, to identify clones from a human PAC library and as a chromosome painting probe in fluorescence in situ hybridization.

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Selected References

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