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. 1995 Oct 25;23(20):4222–4223. doi: 10.1093/nar/23.20.4222

A strategy for quickly identifying all unique two-hybrid or library plasmids within a pool of yeast transformants.

T E Patterson 1, G R Stark 1, S Sazer 1
PMCID: PMC307366  PMID: 7479088

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Church G. M., Gilbert W. Genomic sequencing. Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991–1995. doi: 10.1073/pnas.81.7.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Guerrini A. M., Ascenzioni F., Gallo T., Donini P. Determination of plasmid copy number in yeast transformants by means of agarose plugs. Nucleic Acids Res. 1993 Dec 25;21(25):6047–6048. doi: 10.1093/nar/21.25.6047. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hagan I., Hayles J., Nurse P. Cloning and sequencing of the cyclin-related cdc13+ gene and a cytological study of its role in fission yeast mitosis. J Cell Sci. 1988 Dec;91(Pt 4):587–595. doi: 10.1242/jcs.91.4.587. [DOI] [PubMed] [Google Scholar]
  4. Heyer W. D., Sipiczki M., Kohli J. Replicating plasmids in Schizosaccharomyces pombe: improvement of symmetric segregation by a new genetic element. Mol Cell Biol. 1986 Jan;6(1):80–89. doi: 10.1128/mcb.6.1.80. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Matsumoto T., Beach D. Premature initiation of mitosis in yeast lacking RCC1 or an interacting GTPase. Cell. 1991 Jul 26;66(2):347–360. doi: 10.1016/0092-8674(91)90624-8. [DOI] [PubMed] [Google Scholar]
  6. McIntyre P., Stark G. R. A quantitative method for analyzing specific DNA sequences directly from whole cells. Anal Biochem. 1988 Oct;174(1):209–214. doi: 10.1016/0003-2697(88)90537-4. [DOI] [PubMed] [Google Scholar]
  7. Runge K. W., Zakian V. A. Introduction of extra telomeric DNA sequences into Saccharomyces cerevisiae results in telomere elongation. Mol Cell Biol. 1989 Apr;9(4):1488–1497. doi: 10.1128/mcb.9.4.1488. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Sazer S., Nurse P. A fission yeast RCC1-related protein is required for the mitosis to interphase transition. EMBO J. 1994 Feb 1;13(3):606–615. doi: 10.1002/j.1460-2075.1994.tb06298.x. [DOI] [PMC free article] [PubMed] [Google Scholar]

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