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. 1995 Nov 11;23(21):4262–4266. doi: 10.1093/nar/23.21.4262

The gene for the human architectural transcription factor HMGI-C consists of five exons each coding for a distinct functional element.

K Y Chau 1, U A Patel 1, K L Lee 1, H Y Lam 1, C Crane-Robinson 1
PMCID: PMC307378  PMID: 7501444

Abstract

The gene on chromosome 12 coding for the human protein HMGI-C has been cloned and partially sequenced. It consists of five exons, the first and last of which include long untranslated regions. The 5' UTR includes a (CA/T)n tract and a polymorphic (CT)n tract. Exons II, III and IV (87, 51 and 33 bp) are dispersed over > 30 kb. Exons I-III separately encode the three basic DNA binding domains ('A-T hooks'), exon IV encodes an 11 amino acid sequence characteristic of HMGI-C and absent from the human HMGI(Y) gene [Friedmann, M., Holth, L. T., Zoghbi, H. Y. and Reeves, R. (1993) Nucleic Acids Res., 21, 4259-4267], whilst exon V encodes the acidic C-terminal domain, which is subject to multiple phosphorylation. The HMGI-C gene is thus a striking example of the separation of functional protein elements into different coding exons.

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Selected References

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  1. Ashar H. R., Fejzo M. S., Tkachenko A., Zhou X., Fletcher J. A., Weremowicz S., Morton C. C., Chada K. Disruption of the architectural factor HMGI-C: DNA-binding AT hook motifs fused in lipomas to distinct transcriptional regulatory domains. Cell. 1995 Jul 14;82(1):57–65. doi: 10.1016/0092-8674(95)90052-7. [DOI] [PubMed] [Google Scholar]
  2. Benson K. F., Chada K. Mini-mouse: phenotypic characterization of a transgenic insertional mutant allelic to pygmy. Genet Res. 1994 Aug;64(1):27–33. doi: 10.1017/s0016672300032511. [DOI] [PubMed] [Google Scholar]
  3. Berlingieri M. T., Manfioletti G., Santoro M., Bandiera A., Visconti R., Giancotti V., Fusco A. Inhibition of HMGI-C protein synthesis suppresses retrovirally induced neoplastic transformation of rat thyroid cells. Mol Cell Biol. 1995 Mar;15(3):1545–1553. doi: 10.1128/mcb.15.3.1545. [DOI] [PMC free article] [PubMed] [Google Scholar] [Retracted]
  4. Bird A. P. CpG-rich islands and the function of DNA methylation. Nature. 1986 May 15;321(6067):209–213. doi: 10.1038/321209a0. [DOI] [PubMed] [Google Scholar]
  5. Breathnach R., Chambon P. Organization and expression of eucaryotic split genes coding for proteins. Annu Rev Biochem. 1981;50:349–383. doi: 10.1146/annurev.bi.50.070181.002025. [DOI] [PubMed] [Google Scholar]
  6. Chuvpilo S., Schomberg C., Gerwig R., Heinfling A., Reeves R., Grummt F., Serfling E. Multiple closely-linked NFAT/octamer and HMG I(Y) binding sites are part of the interleukin-4 promoter. Nucleic Acids Res. 1993 Dec 11;21(24):5694–5704. doi: 10.1093/nar/21.24.5694. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Eckner R., Birnstiel M. L. Cloning of cDNAs coding for human HMG I and HMG Y proteins: both are capable of binding to the octamer sequence motif. Nucleic Acids Res. 1989 Aug 11;17(15):5947–5959. doi: 10.1093/nar/17.15.5947. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ferranti P., Malorni A., Marino G., Pucci P., Goodwin G. H., Manfioletti G., Giancotti V. Mass spectrometric analysis of the HMGY protein from Lewis lung carcinoma. Identification of phosphorylation sites. J Biol Chem. 1992 Nov 5;267(31):22486–22489. [PubMed] [Google Scholar]
  9. Friedmann M., Holth L. T., Zoghbi H. Y., Reeves R. Organization, inducible-expression and chromosome localization of the human HMG-I(Y) nonhistone protein gene. Nucleic Acids Res. 1993 Sep 11;21(18):4259–4267. doi: 10.1093/nar/21.18.4259. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Geierstanger B. H., Volkman B. F., Kremer W., Wemmer D. E. Short peptide fragments derived from HMG-I/Y proteins bind specifically to the minor groove of DNA. Biochemistry. 1994 May 3;33(17):5347–5355. doi: 10.1021/bi00183a043. [DOI] [PubMed] [Google Scholar]
  11. Giancotti V., Bandiera A., Ciani L., Santoro D., Crane-Robinson C., Goodwin G. H., Boiocchi M., Dolcetti R., Casetta B. High-mobility-group (HMG) proteins and histone H1 subtypes expression in normal and tumor tissues of mouse. Eur J Biochem. 1993 Apr 15;213(2):825–832. doi: 10.1111/j.1432-1033.1993.tb17825.x. [DOI] [PubMed] [Google Scholar]
  12. Giancotti V., Berlingieri M. T., DiFiore P. P., Fusco A., Vecchio G., Crane-Robinson C. Changes in nuclear proteins on transformation of rat epithelial thyroid cells by a murine sarcoma retrovirus. Cancer Res. 1985 Dec;45(12 Pt 1):6051–6057. [PubMed] [Google Scholar]
  13. Giancotti V., Buratti E., Perissin L., Zorzet S., Balmain A., Portella G., Fusco A., Goodwin G. H. Analysis of the HMGI nuclear proteins in mouse neoplastic cells induced by different procedures. Exp Cell Res. 1989 Oct;184(2):538–545. doi: 10.1016/0014-4827(89)90352-2. [DOI] [PubMed] [Google Scholar]
  14. Giancotti V., Pani B., D'Andrea P., Berlingieri M. T., Di Fiore P. P., Fusco A., Vecchio G., Philp R., Crane-Robinson C., Nicolas R. H. Elevated levels of a specific class of nuclear phosphoproteins in cells transformed with v-ras and v-mos oncogenes and by cotransfection with c-myc and polyoma middle T genes. EMBO J. 1987 Jul;6(7):1981–1987. doi: 10.1002/j.1460-2075.1987.tb02461.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Johnson K. R., Lehn D. A., Elton T. S., Barr P. J., Reeves R. Complete murine cDNA sequence, genomic structure, and tissue expression of the high mobility group protein HMG-I(Y). J Biol Chem. 1988 Dec 5;263(34):18338–18342. [PubMed] [Google Scholar]
  16. Johnson K. R., Lehn D. A., Reeves R. Alternative processing of mRNAs encoding mammalian chromosomal high-mobility-group proteins HMG-I and HMG-Y. Mol Cell Biol. 1989 May;9(5):2114–2123. doi: 10.1128/mcb.9.5.2114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Lanahan A., Williams J. B., Sanders L. K., Nathans D. Growth factor-induced delayed early response genes. Mol Cell Biol. 1992 Sep;12(9):3919–3929. doi: 10.1128/mcb.12.9.3919. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Leger H., Sock E., Renner K., Grummt F., Wegner M. Functional interaction between the POU domain protein Tst-1/Oct-6 and the high-mobility-group protein HMG-I/Y. Mol Cell Biol. 1995 Jul;15(7):3738–3747. doi: 10.1128/mcb.15.7.3738. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Lewis H., Kaszubska W., DeLamarter J. F., Whelan J. Cooperativity between two NF-kappa B complexes, mediated by high-mobility-group protein I(Y), is essential for cytokine-induced expression of the E-selectin promoter. Mol Cell Biol. 1994 Sep;14(9):5701–5709. doi: 10.1128/mcb.14.9.5701. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Manfioletti G., Giancotti V., Bandiera A., Buratti E., Sautière P., Cary P., Crane-Robinson C., Coles B., Goodwin G. H. cDNA cloning of the HMGI-C phosphoprotein, a nuclear protein associated with neoplastic and undifferentiated phenotypes. Nucleic Acids Res. 1991 Dec 25;19(24):6793–6797. doi: 10.1093/nar/19.24.6793. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. McLauchlan J., Gaffney D., Whitton J. L., Clements J. B. The consensus sequence YGTGTTYY located downstream from the AATAAA signal is required for efficient formation of mRNA 3' termini. Nucleic Acids Res. 1985 Feb 25;13(4):1347–1368. doi: 10.1093/nar/13.4.1347. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Patel U. A., Bandiera A., Manfioletti G., Giancotti V., Chau K. Y., Crane-Robinson C. Expression and cDNA cloning of human HMGI-C phosphoprotein. Biochem Biophys Res Commun. 1994 May 30;201(1):63–70. doi: 10.1006/bbrc.1994.1669. [DOI] [PubMed] [Google Scholar]
  23. Reeves R., Nissen M. S. The A.T-DNA-binding domain of mammalian high mobility group I chromosomal proteins. A novel peptide motif for recognizing DNA structure. J Biol Chem. 1990 May 25;265(15):8573–8582. [PubMed] [Google Scholar]
  24. Schoenmakers E. F., Wanschura S., Mols R., Bullerdiek J., Van den Berghe H., Van de Ven W. J. Recurrent rearrangements in the high mobility group protein gene, HMGI-C, in benign mesenchymal tumours. Nat Genet. 1995 Aug;10(4):436–444. doi: 10.1038/ng0895-436. [DOI] [PubMed] [Google Scholar]
  25. Sreekantaiah C., Leong S. P., Karakousis C. P., McGee D. L., Rappaport W. D., Villar H. V., Neal D., Fleming S., Wankel A., Herrington P. N. Cytogenetic profile of 109 lipomas. Cancer Res. 1991 Jan 1;51(1):422–433. [PubMed] [Google Scholar]
  26. Thanos D., Maniatis T. The high mobility group protein HMG I(Y) is required for NF-kappa B-dependent virus induction of the human IFN-beta gene. Cell. 1992 Nov 27;71(5):777–789. doi: 10.1016/0092-8674(92)90554-p. [DOI] [PubMed] [Google Scholar]
  27. Tkachuk D. C., Kohler S., Cleary M. L. Involvement of a homolog of Drosophila trithorax by 11q23 chromosomal translocations in acute leukemias. Cell. 1992 Nov 13;71(4):691–700. doi: 10.1016/0092-8674(92)90602-9. [DOI] [PubMed] [Google Scholar]
  28. Xiang X., Benson K. F., Chada K. Mini-mouse: disruption of the pygmy locus in a transgenic insertional mutant. Science. 1990 Feb 23;247(4945):967–969. doi: 10.1126/science.2305264. [DOI] [PubMed] [Google Scholar]

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