Skip to main content
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1995 Nov 11;23(21):4518–4519. doi: 10.1093/nar/23.21.4518

Rapid purification of recombinant Taq DNA polymerase by freezing and high temperature thawing of bacterial expression cultures.

E Grimm 1, P Arbuthnot 1
PMCID: PMC307412  PMID: 7501478

Abstract

Full text

PDF

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Engelke D. R., Krikos A., Bruck M. E., Ginsburg D. Purification of Thermus aquaticus DNA polymerase expressed in Escherichia coli. Anal Biochem. 1990 Dec;191(2):396–400. doi: 10.1016/0003-2697(90)90238-5. [DOI] [PubMed] [Google Scholar]
  2. Pluthero F. G. Rapid purification of high-activity Taq DNA polymerase. Nucleic Acids Res. 1993 Oct 11;21(20):4850–4851. doi: 10.1093/nar/21.20.4850. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Studier F. W., Rosenberg A. H., Dunn J. J., Dubendorff J. W. Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol. 1990;185:60–89. doi: 10.1016/0076-6879(90)85008-c. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES