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. 1995 Dec 11;23(23):4928–4929. doi: 10.1093/nar/23.23.4928

A simple and rapid electrophoresis method to detect sequence variation in PCR-amplified DNA fragments.

C Wawer 1, H Rüggeberg 1, G Meyer 1, G Muyzer 1
PMCID: PMC307487  PMID: 8532541

Abstract

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Deckers H. M., Wilson F. R., Voordouw G. Cloning and sequencing of a [NiFe] hydrogenase operon from Desulfovibrio vulgaris Miyazaki F. J Gen Microbiol. 1990 Oct;136(10):2021–2028. doi: 10.1099/00221287-136-10-2021. [DOI] [PubMed] [Google Scholar]
  2. Fischer S. G., Lerman L. S. DNA fragments differing by single base-pair substitutions are separated in denaturing gradient gels: correspondence with melting theory. Proc Natl Acad Sci U S A. 1983 Mar;80(6):1579–1583. doi: 10.1073/pnas.80.6.1579. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Kruse L., Meyer G., Hildebrandt A. A highly conserved repetitive sequence from Physarum polycephalum contains nucleotide arrangements similar to replicator sequences. Biochim Biophys Acta. 1993 Oct 19;1216(1):129–133. doi: 10.1016/0167-4781(93)90049-j. [DOI] [PubMed] [Google Scholar]
  4. Li C., Peck H. D., Jr, LeGall J., Przybyla A. E. Cloning, characterization, and sequencing of the genes encoding the large and small subunits of the periplasmic [NiFe]hydrogenase of Desulfovibrio gigas. DNA. 1987 Dec;6(6):539–551. doi: 10.1089/dna.1987.6.539. [DOI] [PubMed] [Google Scholar]
  5. Müller W., Gautier F. Interactions of heteroaromatic compounds with nucleic acids. A - T-specific non-intercalating DNA ligands. Eur J Biochem. 1975 Jun;54(2):385–394. doi: 10.1111/j.1432-1033.1975.tb04149.x. [DOI] [PubMed] [Google Scholar]
  6. Müller W., Hattesohl I., Schuetz H. J., Meyer G. Polyethylene glycol derivatives of base and sequence specific DNA ligands: DNA interaction and application for base specific separation of DNA fragments by gel electrophoresis. Nucleic Acids Res. 1981 Jan 10;9(1):95–119. doi: 10.1093/nar/9.1.95. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Orita M., Iwahana H., Kanazawa H., Hayashi K., Sekiya T. Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms. Proc Natl Acad Sci U S A. 1989 Apr;86(8):2766–2770. doi: 10.1073/pnas.86.8.2766. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Rosenbaum V., Riesner D. Temperature-gradient gel electrophoresis. Thermodynamic analysis of nucleic acids and proteins in purified form and in cellular extracts. Biophys Chem. 1987 May 9;26(2-3):235–246. doi: 10.1016/0301-4622(87)80026-1. [DOI] [PubMed] [Google Scholar]
  9. Rousset M., Dermoun Z., Hatchikian C. E., Bélaich J. P. Cloning and sequencing of the locus encoding the large and small subunit genes of the periplasmic [NiFe]hydrogenase from Desulfovibrio fructosovorans. Gene. 1990 Sep 28;94(1):95–101. doi: 10.1016/0378-1119(90)90473-5. [DOI] [PubMed] [Google Scholar]
  10. STUDIER F. W. SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA. J Mol Biol. 1965 Feb;11:373–390. doi: 10.1016/s0022-2836(65)80064-x. [DOI] [PubMed] [Google Scholar]
  11. Wawer C., Muyzer G. Genetic diversity of Desulfovibrio spp. in environmental samples analyzed by denaturing gradient gel electrophoresis of [NiFe] hydrogenase gene fragments. Appl Environ Microbiol. 1995 Jun;61(6):2203–2210. doi: 10.1128/aem.61.6.2203-2210.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]

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