Fig. 4.

Effect of dutasteride on the expression of indicated androgen-responsive genes. Tumor bearing mice were castrated when the tumors reached a volume of 100 mm³.After castration, they were randomized to C,C+D,T, or T+D.TIC and TIC+D mice were followed up concurrently. Transcript levels for the assayed genes were normalized to GAPDH using the ΔCP method. Each dot represents a single sample, and the horizontal line depicts the median. PSA (**P < 0.01) and U19/Eaf2 (*P < 0.05) expression was significantly decreased in castrated mice when compared to TIC. Expression of PSA, U19/Eaf2, Calreticulin and ADI1 was significantly decreased, **P < 0.01, in TIC mice treated with D. Expression of U19/Eaf2 and calreticulin was enhanced in the T+D group, **P < 0.01. Unpaired t-test was used for P-value calculation.