Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2011 Oct 1.

Published in final edited form as: Leukemia. 2011 Jan 25;25(4):655–662. doi: 10.1038/leu.2010.301

Schematic representation of viral vectors (A) and experimental strategy (B) used in BM transplantation assay. Hematopoietic progenitors from wild type and Cbfb^+/56M;Mx1Cre mice (expressing CBFb-MYH11) were infected with MIG, MIG-Mpl, or MIG-Mpl-Y112F retrovirus and transplanted into sub-lethally irradiated recipient mice. (C) Kaplan Meier survival curves for mice transplanted with wild type (wt) or Cbfb^+/56M;Mx1Cre (CM) cells infected with MIG-Mpl (Mpl), MIG-MplY112, or MIG retroviruses. (D) Representative FACS analysis of peripheral blood white blood cells from a leukemic mouse expressing Cbfβ-SMMHC and MIG-Mpl (GFP+cKit+ cells). (E) Representative cell morphology of 2 AML samples from peripheral blood of CM/Mpl leukemic mice, depicting balstlike (red arrow) and monocyticlike (asterisks), with magnification 100X. (F) Spleen weight of leukemic (n=11) and control (n=6) mice.

Schematic representation of viral vectors (A) and experimental strategy (B) used in BM transplantation assay. Hematopoietic progenitors from wild type and Cbfb^+/56M;Mx1Cre mice (expressing CBFb-MYH11) were infected with MIG, MIG-Mpl, or MIG-Mpl-Y112F retrovirus and transplanted into sub-lethally irradiated recipient mice. (C) Kaplan Meier survival curves for mice transplanted with wild type (wt) or Cbfb^+/56M;Mx1Cre (CM) cells infected with MIG-Mpl (Mpl), MIG-MplY112, or MIG retroviruses. (D) Representative FACS analysis of peripheral blood white blood cells from a leukemic mouse expressing Cbfβ-SMMHC and MIG-Mpl (GFP+cKit+ cells). (E) Representative cell morphology of 2 AML samples from peripheral blood of CM/Mpl leukemic mice, depicting balstlike (red arrow) and monocyticlike (asterisks), with magnification 100X. (F) Spleen weight of leukemic (n=11) and control (n=6) mice.