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. 1994 Feb 25;22(4):632–638. doi: 10.1093/nar/22.4.632

DNA ligase I is associated with the 21 S complex of enzymes for DNA synthesis in HeLa cells.

C Li 1, J Goodchild 1, E F Baril 1
PMCID: PMC307854  PMID: 8127711

Abstract

Approximately 80% of the DNA ligase activity in HeLa cell extracts is associated with the 21 S enzyme complex that functions in simian virus 40 DNA replication in vitro (Malkas et al., Biochemistry 29, 6362-6374., 1990). The DNA ligase associated with the 21 S complex was purified extensively and its physical, enzymic and immunological properties characterized as DNA ligase I. The association of DNA ligase I with the 21 S complex of enzymes for DNA synthesis provides evidence for the physiological function of this DNA ligase in DNA replication in human cells.

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Selected References

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