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. 1994 Feb 25;22(4):639–645. doi: 10.1093/nar/22.4.639

On the rapid deprotection of synthetic oligonucleotides and analogs.

N N Polushin 1, A M Morocho 1, B C Chen 1, J S Cohen 1
PMCID: PMC307855  PMID: 8127712

Abstract

The efficiency of oligodeoxynucleotide deprotection is greatly enhanced using a combination of: (a) ethanolamine, and especially a mixture of hydrazine, ethanolamine and methanol, in place of the usual aqueous ammonia; (b) tert-butylphenoxyacetyl amino protecting groups, and (c) oxalyl link between the first nucleotide and the polymeric support. The extent of base modification, particularly of C, is shown to be extremely low, and the quality of deprotected oligonucleotides is as high as in the case of ammonia deprotection. This method is also shown to be applicable to the preparation of phosphorothioate and methylphosphonate oligodeoxynucleotides and oligoribonucleotides.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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