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. 1994 Feb 25;22(4):695–696. doi: 10.1093/nar/22.4.695

Methylation analysis on individual chromosomes: improved protocol for bisulphite genomic sequencing.

R Feil 1, J Charlton 1, A P Bird 1, J Walter 1, W Reik 1
PMCID: PMC307863  PMID: 8127720

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Selected References

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  1. Frommer M., McDonald L. E., Millar D. S., Collis C. M., Watt F., Grigg G. W., Molloy P. L., Paul C. L. A genomic sequencing protocol that yields a positive display of 5-methylcytosine residues in individual DNA strands. Proc Natl Acad Sci U S A. 1992 Mar 1;89(5):1827–1831. doi: 10.1073/pnas.89.5.1827. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Hayatsu H. Bisulfite modification of nucleic acids and their constituents. Prog Nucleic Acid Res Mol Biol. 1976;16:75–124. doi: 10.1016/s0079-6603(08)60756-4. [DOI] [PubMed] [Google Scholar]
  3. Hayatsu H., Wataya Y., Kai K., Iida S. Reaction of sodium bisulfite with uracil, cytosine, and their derivatives. Biochemistry. 1970 Jul 7;9(14):2858–2865. doi: 10.1021/bi00816a016. [DOI] [PubMed] [Google Scholar]
  4. Shapiro R., DiFate V., Welcher M. Deamination of cytosine derivatives by bisulfite. Mechanism of the reaction. J Am Chem Soc. 1974 Feb 6;96(3):906–912. doi: 10.1021/ja00810a043. [DOI] [PubMed] [Google Scholar]
  5. Shortle D., Nathans D. Local mutagenesis: a method for generating viral mutants with base substitutions in preselected regions of the viral genome. Proc Natl Acad Sci U S A. 1978 May;75(5):2170–2174. doi: 10.1073/pnas.75.5.2170. [DOI] [PMC free article] [PubMed] [Google Scholar]

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