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. Author manuscript; available in PMC: 2012 Feb 8.
Published in final edited form as: Biochemistry. 2011 Jan 13;50(5):612–617. doi: 10.1021/bi101536k

Table 1.

Rate and equilibrium constants for DNA base-openinga

Kinetic Parameter T/Ab U/A 5-FU/A
kex (s−1) 0.63 ± 0.02 6.3 ± 0.1 126 ± 8
kint × 10−6 (s−1) 0.02 ± 0.03 0.4 ± 0.2 14 ± 3
kop (s−1) 35 ± 6 200 ± 25 440 ± 20
kcl × 10−6 (s−1) 1.8 ± 0.3 7 ± 3 37 ± 7
αKop × 106 20 ± 4 27 ± 11 12 ± 2
a

Abbreviations: 5-FU, 5-fluorouracil; U, uracil; A, adenine; T, thymine.

b

Kinetic parameters for the T•A base-pair were previously reported (13).