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Room temperature time course of the reaction of oxy-DosT with 1.33 equiv of DEA-NONOate at pH 7.4 (DEA-NONOate generates 1.5 equiv NO with a t1/2 ∼ 16 min). Panel A shows the first 21 minutes of the reaction while panel B corresponds to the subsequent 30 minutes. Reference spectra of oxy-DosT (A) and DosT Fe(III)-NO (B) are also shown (black dashed lines).
