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Binding of individual phage clones harboring scFv fragments to HCV core protein. The phage population after three or four rounds of panning was diluted, and single phages were amplified. Their reactivity with core protein (aa 1-115) relative to BSA was determined by phage EIA. Clones scFv41C, scFv42C, scFv50C, and scFv29C were thus identified as core protein binders.
