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. 1994 May 11;22(9):1675–1680. doi: 10.1093/nar/22.9.1675

The promoter of the Xwnt-5C gene contains octamer and AP-2 motifs functional in Xenopus embryos.

G A Kuiken 1, P J Bertens 1, J Peterson-Maduro 1, G J Veenstra 1, J G Koster 1, O H Destrée 1
PMCID: PMC308048  PMID: 8202371

Abstract

The Xwnt-5C gene is expressed in Xenopus embryos from the early gastrula stage onwards. The transcription of Xwnt-5C is regulated differentially with respect to transcript size, timing and localization. To gain insight into the generation of the Xwnt-5C expression pattern, we started to analyze the transcriptional regulation of this gene. We isolated Xwnt-5C genomic DNA sequences. By microinjection of chimaeric reporter constructs into Xenopus embryos we demonstrate that the upstream region contains a promoter functional in vivo. Of the several putative binding sites for trans-acting factors, present in a minimal promoter fragment, some have been studied in more detail. Mutations in an octamer motif and in an AP-2 consensus sequence interfere with the activity of the Xwnt-5C minimal promoter. In vitro binding assays with extracts from gastrula stage Xenopus embryos show that the octamer motif of the Xwnt-5C promoter can bind several Octamer binding factors, one of which is Oct1.

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Selected References

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