Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1994 May 25;22(10):1925–1927. doi: 10.1093/nar/22.10.1925

RNA recovery efficiency for cultured cells and organ-derived cell suspensions.

E S Berleth 1, B J Dolnick 1, M J Ehrke 1, E Mihich 1
PMCID: PMC308098  PMID: 7516067

Abstract

Full text

PDF
1925

Images in this article

1925Image
on p.1925

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alwine J. C., Kemp D. J., Stark G. R. Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5350–5354. doi: 10.1073/pnas.74.12.5350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Berk A. J., Sharp P. A. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids. Cell. 1977 Nov;12(3):721–732. doi: 10.1016/0092-8674(77)90272-0. [DOI] [PubMed] [Google Scholar]
  3. Brenner C. A., Tam A. W., Nelson P. A., Engleman E. G., Suzuki N., Fry K. E., Larrick J. W. Message amplification phenotyping (MAPPing): a technique to simultaneously measure multiple mRNAs from small numbers of cells. Biotechniques. 1989 Nov-Dec;7(10):1096–1103. [PubMed] [Google Scholar]
  4. Chirgwin J. M., Przybyla A. E., MacDonald R. J., Rutter W. J. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979 Nov 27;18(24):5294–5299. doi: 10.1021/bi00591a005. [DOI] [PubMed] [Google Scholar]
  5. Dallman M. J., Montgomery R. A., Larsen C. P., Wanders A., Wells A. F. Cytokine gene expression: analysis using northern blotting, polymerase chain reaction and in situ hybridization. Immunol Rev. 1991 Feb;119:163–179. doi: 10.1111/j.1600-065x.1991.tb00583.x. [DOI] [PubMed] [Google Scholar]
  6. Darzynkiewicz Z., Traganos F., Sharpless T., Melamed M. R. Lymphocyte stimulation: a rapid multiparameter analysis. Proc Natl Acad Sci U S A. 1976 Aug;73(8):2881–2884. doi: 10.1073/pnas.73.8.2881. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. DuBois D. C., Almon R. R., Jusko W. J. Molar quantification of specific messenger ribonucleic acid expression in northern hybridization using cRNA standards. Anal Biochem. 1993 Apr;210(1):140–144. doi: 10.1006/abio.1993.1164. [DOI] [PubMed] [Google Scholar]
  8. Ehrke M. J., Ryoyama K., Cohen S. A. Cellular basis for adriamycin-induced augmentation of cell-mediated cytotoxicity in culture. Cancer Res. 1984 Jun;44(6):2497–2504. [PubMed] [Google Scholar]
  9. Guillem J. G., Levy M. F., Hsieh L. L., Johnson M. D., LoGerfo P., Forde K. A., Weinstein I. B. Increased levels of phorbin, c-myc, and ornithine decarboxylase RNAs in human colon cancer. Mol Carcinog. 1990;3(2):68–74. doi: 10.1002/mc.2940030204. [DOI] [PubMed] [Google Scholar]
  10. Hengerer B. A rapid procedure for mRNA extraction from a large number of samples. Biotechniques. 1993 Apr;14(4):522–524. [PubMed] [Google Scholar]
  11. Heumann R., Thoenen H. Comparison between the time course of changes in nerve growth factor protein levels and those of its messenger RNA in the cultured rat iris. J Biol Chem. 1986 Jul 15;261(20):9246–9249. [PubMed] [Google Scholar]
  12. Toscani A., Soprano D. R., Cosenza S. C., Owen T. A., Soprano K. J. Normalization of multiple RNA samples using an in vitro-synthesized external standard cRNA. Anal Biochem. 1987 Sep;165(2):309–319. doi: 10.1016/0003-2697(87)90274-0. [DOI] [PubMed] [Google Scholar]
  13. Wallen C. A., Higashikubo R., Dethlefsen L. A. Comparison of two flow cytometric assays for cellular RNA--acridine orange and propidium iodide. Cytometry. 1982 Nov;3(3):155–160. doi: 10.1002/cyto.990030303. [DOI] [PubMed] [Google Scholar]
  14. Woloschak G. E., Chang-Liu C. M., Jones P. S., Jones C. A. Modulation of gene expression in Syrian hamster embryo cells following ionizing radiation. Cancer Res. 1990 Jan 15;50(2):339–344. [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES