Table 1.

Cytochrome c yields in milligrams of protein per gram of wet cells. Maturation was accomplished using either yeast cytochrome c heme lyase (CCHL) or the cytochrome c maturation (CCM) system.

	Horse cyt c		Ht cyt c
Variant	CCHL	CCM	CCM
1Xa	N.D.	N.D.	0.23
WT	1.03	0.60	2.93
3Xb	0.27	0.77	2.62
4Xc	-	-	2.73
G13ins	0.3d	0.68	-
F10A	N.D.	0.30	-

^aCGCH heme attachment sequence for both horse and Ht cyt c.

^bCAQGCH for horse and CMAGCH for Ht cyt c.

^cCAAQGCH for horse and CAMAGCH for Ht cyt c.

^dHeterogeneous product; presence of correctly matured protein is uncertain.

^eN.D. indicates that expressed cytochrome was not detected.