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. 1994 Jul 11;22(13):2460–2465. doi: 10.1093/nar/22.13.2460

Comparison of IR- and UV-matrix-assisted laser desorption/ionization mass spectrometry of oligodeoxynucleotides.

E Nordhoff 1, F Kirpekar 1, M Karas 1, R Cramer 1, S Hahner 1, F Hillenkamp 1, K Kristiansen 1, P Roepstroff 1, A Lezius 1
PMCID: PMC308196  PMID: 8041606

Abstract

UV-matrix assisted laser desorption/ionization mass spectrometry (UV-MALDI-MS) with 3-hydroxypicolinic acid as matrix and IR-MALDI-MS with succinic acid as matrix have proved their feasibility for highly accurate and sensitive mass determination of nucleic acids (DNA and RNA). In this work, a detailed comparison of these two MALDI-methods and between positive- and negative ion mass spectra for the analysis of oligodeoxynucleotides is undertaken. Mass spectra of DNA sequences with up to 40 nucleotides are shown. Both linear and reflectron time-of-flight mass analyzers were used within this study and are compared for their potential in the MALDI analysis of oligodeoxynucleotides. The role of molecule-ion fragmentation is also discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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