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. 1994 Jul 11;22(13):2667–2669. doi: 10.1093/nar/22.13.2667

Sequencing of oligonucleotide phosphorothioates based on solid-supported desulfurization.

T K Wyrzykiewicz 1, D L Cole 1
PMCID: PMC308225  PMID: 8041629

Abstract

We described a solid-supported desulfurization procedure allowing easy access to the sequence analysis of oligonucleotide phosphorothioates. The described method is based upon selective removal of the 2-cyanoethyl phosphate protecting groups, followed by iodine-promoted desulfurization of the resulting phosphorothioate diesters. Automatic oxidation of oligonucleotide phosphorothioates, anchored via an ester linkage to a standard solid support (LCAA/CPG), is combined with Maxam-Gilbert solid-support sequencing. The overall procedure allows rapid simultaneous sequence analysis of several oligonucleotide analogs.

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Selected References

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