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. 1994 Sep 11;22(18):3689–3692. doi: 10.1093/nar/22.18.3689

Fluorescence in situ hybridisation of multiple probes on a single microscope slide.

Z Larin 1, M D Fricker 1, E Maher 1, Y Ishikawa-Brush 1, E M Southern 1
PMCID: PMC308347  PMID: 7937078

Abstract

We report a method to analyse multiple samples by fluorescence in situ hybridisation on a single glass microscope slide. Wells were formed in which independent hybridisation reactions could proceed by sealing a silicon rubber gasket to the slide. In the largest format tested, different probes were hybridised simultaneously by applying them directly from a 96-well microtitre dish which was inverted on a glass plate. This technique will increase the rate of analysis of multiple probes against a standard set of chromosomes and could also be used to analyse different karyotypes using a panel of probes such as single chromosome paints during a single operation. It should be useful for both chromosomal mapping projects and screening for chromosome abnormalities in clinical diagnostic laboratories.

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Selected References

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