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. 1994 Sep 25;22(19):3866–3870. doi: 10.1093/nar/22.19.3866

Matrix assisted laser desorption/ionization mass spectrometry of enzymatically synthesized RNA up to 150 kDa.

F Kirpekar 1, E Nordhoff 1, K Kristiansen 1, P Roepstorff 1, A Lezius 1, S Hahner 1, M Karas 1, F Hillenkamp 1
PMCID: PMC308382  PMID: 7524029

Abstract

Enzymatically synthesized RNA samples (in vitro transcripts) were analysed by matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS). Spectra of RNA up to 150 kDA (461 nucleotides) are shown. Polymerase generated sample heterogeneity and its contribution to mass resolution are discussed. A time course exonuclease digest of a 55 nt in vitro transcript was analyzed to investigate the performance of MALDI-MS on complex mixtures. Based on these data, the analysis by MALDI-MS of DNA sequencing reactions, produced by the action of an RNA polymerase, is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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