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. 1994 Sep 25;22(19):3928–3929. doi: 10.1093/nar/22.19.3928

New restriction endonuclease CviRI cleaves DNA at TG/CA sequences.

A Jin 1, Y Zhang 1, Y Xia 1, E Traylor 1, M Nelson 1, J L Van Etten 1
PMCID: PMC308391  PMID: 7937114

Abstract

A new type II restriction endonuclease, CviRI, was isolated from virus XZ-6E infected chlorella cells. CviRI is the first restriction endonuclease to recognize the sequence 5'-TGCA-3' and cleaves DNA between the G and C residues to produce blunt-end termini. Methylation of the adenine or cytosine in 5'-TGCA-3' sequences prevents CviRI cleavage. Due to its sequence specificity, CviRI may be especially useful for detecting mutant alleles of many heritable human genetic diseases.

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Selected References

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