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. Author manuscript; available in PMC: 2012 Apr 26.
Published in final edited form as: Biochemistry. 2011 Mar 30;50(16):3394–3403. doi: 10.1021/bi200197e

Figure 1.

Figure 1

Purification of myristoylated SF2 Nef and h-NMT-1. (A) The MyrNef:NMT complex copurified in gel filtration as both species were seen in the mass spectrum (see also Supporting Information Figures 1, 2). (B) After the MyrNef:NMT complex was rebound to Ni-affinity beads, NMT was eluted with the addition of 10 μM myrCoA. (C) MyrNef was eluted from the Ni-affinity beads with imidazole. In these electrospray mass spectra, the masses of MyrNef (25553.5 Da) and NMT (48140.4 Da) match their theoretical mass values of 25554.0 Da and 48140.8 Da, respectively.