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. Author manuscript; available in PMC: 2011 May 3.
Published in final edited form as: Proteomics. 2010 Apr;10(7):1359–1373. doi: 10.1002/pmic.200900483

Fig. 1. Outline of the quantitative proteomic strategy using 4-plex iTRAQ reagents.

Fig. 1

iTRAQ labeling was carried out separately using whole cell lysate, cytosolic or non-cytosolic fractions. Samples were digested using trypsin in duplicate and labeled using iTRAQ reagents. Peptides from ECCs were labeled with iTRAQ reagent having 114 and 115 reporters and peptides from ESCs were labeled with iTRAQ reagent having 116 and 117 reporters. After labeling, peptides from all four samples were combined and fractionated by strong cation exchange (SCX) chromatography. Each fraction was then analyzed by LC-MS/MS on a quadrupole time of flight mass spectrometer.