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. Author manuscript; available in PMC: 2012 Apr 19.
Published in final edited form as: Biochemistry. 2011 Mar 29;50(15):3181–3192. doi: 10.1021/bi1019622

Figure 3. CCG-50014 is an irreversible inhibitor of RGS4 and RGS8 and its effects are partially reversed by the thiol reductant DTT.

Figure 3

A) RGS4 and B) RGS8 containing beads were treated for 15 minutes with 100 μM CCG-50014 prior to vigorous washing to remove any unbound compound. To determine if the compound was reacting in a thiol-sensitive manner, washing was performed in the absence or presence of 1 mM DTT. Data are presented as the mean±SEM from at least three independent experiments.