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. 1993 Jan 25;21(2):185–190. doi: 10.1093/nar/21.2.185

Cross-ligation and exchange reactions catalyzed by hairpin ribozymes.

Y Komatsu 1, M Koizumi 1, A Sekiguchi 1, E Ohtsuka 1
PMCID: PMC309090  PMID: 8441626

Abstract

The negative strand of the satellite RNA of tobacco ringspot virus (sTobRV(-)) contains a hairpin catalytic domain that shows self-cleavage and self-ligation activities in the presence of magnesium ions. We describe here that the minimal catalytic domain can catalyze a cross-ligation reaction between two kinds of substrates in trans. The cross-ligated product increased when the reaction temperature was decreased during the reaction from 37 degrees C to 4 degrees C. A two-stranded hairpin ribozyme, divided into two fragments between G45 and U46 in a hairpin loop, showed higher ligation activity than the nondivided ribozyme. The two stranded ribozyme also catalyzed an exchange reaction of the 3'-portion of the cleavage site.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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