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In vitro analysis of Hand2 repression of the Dlx5/6 arch specific enhancer. An I56i-firefly luciferase vector was transfected into MC3T3-E1 cells along with other cDNAs and a renilla reporter construct (efficiency control). All conditions were normalized to corresponding empty vectors of gene expression constructs. Error bars represent s.e.m. Control, representative empty vector; ***P<0.0005; **P<0.005; *P<0.05; n.s., not significant (P>0.1).
