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. 1993 Feb 11;21(3):783. doi: 10.1093/nar/21.3.783

A 'one tube reaction' for synthesis and amplification of total cDNA from small numbers of cells.

R H Don 1, P T Cox 1, J S Mattick 1
PMCID: PMC309203  PMID: 8441705

Abstract

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Belyavsky A., Vinogradova T., Rajewsky K. PCR-based cDNA library construction: general cDNA libraries at the level of a few cells. Nucleic Acids Res. 1989 Apr 25;17(8):2919–2932. doi: 10.1093/nar/17.8.2919. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987 Apr;162(1):156–159. doi: 10.1006/abio.1987.9999. [DOI] [PubMed] [Google Scholar]
  3. McClelland M., Hanish J., Nelson M., Patel Y. KGB: a single buffer for all restriction endonucleases. Nucleic Acids Res. 1988 Jan 11;16(1):364–364. doi: 10.1093/nar/16.1.364. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Wang Z., Brown D. D. A gene expression screen. Proc Natl Acad Sci U S A. 1991 Dec 15;88(24):11505–11509. doi: 10.1073/pnas.88.24.11505. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Welsh J., Liu J. P., Efstratiadis A. Cloning of PCR-amplified total cDNA: construction of a mouse oocyte cDNA library. Genet Anal Tech Appl. 1990 Feb;7(1):5–17. doi: 10.1016/0735-0651(90)90038-h. [DOI] [PubMed] [Google Scholar]

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