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. 1993 Feb 25;21(4):1031–1034. doi: 10.1093/nar/21.4.1031

Two non-histone proteins are associated with the promoter region and histone HI with the transcribed region of active hsp-70 genes as revealed by UV-induced DNA-protein crosslinking in vivo.

S V Belikov 1, A I Belgovsky 1, O V Preobrazhenskaya 1, V L Karpov 1, A D Mirzabekov 1
PMCID: PMC309240  PMID: 8451170

Abstract

We described here an approach for mapping proteins on any sequence of genomic DNA. UV-induced DNA-protein crosslinking within whole cells and the 'protein image' hybridization technique (1) were applied to test the proteins bound to different regions of the D. melanogaster hsp-70 gene. The histone H1-DNA association with the coding region is shown to be maintained, even during very intensive transcription, but is absent in the promoter. Two non-histone proteins with apparent molecular masses of 50 kD (p50) and 100 kD (p100) are crosslinked only to the active hsp-70 gene regulatory region and preferentially bind to its complementary and coding DNA strands, respectively.

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Selected References

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