Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1993 Mar 11;21(5):1213–1217. doi: 10.1093/nar/21.5.1213

Large scale, liquid phase synthesis of oligonucleotides by the phosphoramidite approach.

G M Bonora 1, G Biancotto 1, M Maffini 1, C L Scremin 1
PMCID: PMC309284  PMID: 8464706

Abstract

A new method for the liquid phase synthesis of oligonucleotides is described which makes use of polyethylene glycol (PEG) as soluble support and phosphoramidite derivatives as synthons. The new synthetic protocol was applied to a quite large scale production (about 100 mumoles) of such compounds up to the 20mer level. This solution method, called HELP High Efficiency Liquid Phase) Plus, appears effective in terms of speed and coupling yield and can be evaluated for the production of large amount of oligonucleotides.

Full text

PDF
1213

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bonora G. M., Biancotto G., Scremin C. L. Use of the amidite chemistry in the PEG-supported, large-scale synthesis of oligonucleotides. The 'HELP Plus' method. Nucleic Acids Symp Ser. 1991;(24):222–222. [PubMed] [Google Scholar]
  2. Bonora G. M., Scremin C. L., Colonna F. P., Garbesi A. HELP (high efficiency liquid phase) new oligonucleotide synthesis on soluble polymeric support. Nucleic Acids Res. 1990 Jun 11;18(11):3155–3159. doi: 10.1093/nar/18.11.3155. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Duncan R. Drug-polymer conjugates: potential for improved chemotherapy. Anticancer Drugs. 1992 Jun;3(3):175–210. doi: 10.1097/00001813-199206000-00001. [DOI] [PubMed] [Google Scholar]
  4. Eadie J. S., McBride L. J., Efcavitch J. W., Hoff L. B., Cathcart R. High-performance liquid chromatographic analysis of oligodeoxyribonucleotide base composition. Anal Biochem. 1987 Sep;165(2):442–447. doi: 10.1016/0003-2697(87)90294-6. [DOI] [PubMed] [Google Scholar]
  5. Efimov V. A., Reverdatto S. V., Chakhmakhcheva O. G. New effective method for the synthesis of oligonucleotides via phosphotriester intermediates. Nucleic Acids Res. 1982 Nov 11;10(21):6675–6694. doi: 10.1093/nar/10.21.6675. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. LETSINGER R. L., MAHADEVAN V. OLIGONUCLEOTIDE SYNTHESIS ON A POLYMER SUPPORT. J Am Chem Soc. 1965 Aug 5;87:3526–3527. doi: 10.1021/ja01093a058. [DOI] [PubMed] [Google Scholar]
  7. Pon R. T., Damha M. J., Ogilvie K. K. Modification of guanine bases by nucleoside phosphoramidite reagents during the solid phase synthesis of oligonucleotides. Nucleic Acids Res. 1985 Sep 25;13(18):6447–6465. doi: 10.1093/nar/13.18.6447. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Zon G. Oligonucleotide analogues as potential chemotherapeutic agents. Pharm Res. 1988 Sep;5(9):539–549. doi: 10.1023/a:1015985728434. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES