Abstract
There exist about 1000-1500 internal telomeric sequences per haploid genome in the polytene chromosomes of the hypotrichous ciliate Stylonychia lemnae. All these telomeric repeats are contained in a very conserved element. This element consists of two 2 kb direct repeats flanking a 2.6 kb sequence. Immediately adjacent to one of the repeats a 18mer C4A4C4A4C2 telomeric sequence is localized. Sequences homologous to macronuclear DNA follow 180 bp downstream of the C4A4-bloc. These macronuclear homologous sequences are flanked by the second direct repeat. The possible origin and function of these telomere containing elements is discussed.
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- Ammermann D., Steinbrück G., von Berger L., Hennig W. The development of the macronucleus in the ciliated protozoan Stylonychia mytilus. Chromosoma. 1974 May 10;45(4):401–429. doi: 10.1007/BF00283386. [DOI] [PubMed] [Google Scholar]
- Baird S. E., Fino G. M., Tausta S. L., Klobutcher L. A. Micronuclear genome organization in Euplotes crassus: a transposonlike element is removed during macronuclear development. Mol Cell Biol. 1989 Sep;9(9):3793–3807. doi: 10.1128/mcb.9.9.3793. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Feinberg A. P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. doi: 10.1016/0003-2697(83)90418-9. [DOI] [PubMed] [Google Scholar]
- Finnegan D. J. Transposable elements in eukaryotes. Int Rev Cytol. 1985;93:281–326. doi: 10.1016/s0074-7696(08)61376-5. [DOI] [PubMed] [Google Scholar]
- Glisin V., Crkvenjakov R., Byus C. Ribonucleic acid isolated by cesium chloride centrifugation. Biochemistry. 1974 Jun 4;13(12):2633–2637. doi: 10.1021/bi00709a025. [DOI] [PubMed] [Google Scholar]
- Greider C. W. Telomerase is processive. Mol Cell Biol. 1991 Sep;11(9):4572–4580. doi: 10.1128/mcb.11.9.4572. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Greslin A. F., Prescott D. M., Oka Y., Loukin S. H., Chappell J. C. Reordering of nine exons is necessary to form a functional actin gene in Oxytricha nova. Proc Natl Acad Sci U S A. 1989 Aug;86(16):6264–6268. doi: 10.1073/pnas.86.16.6264. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Herrick G., Cartinhour S., Dawson D., Ang D., Sheets R., Lee A., Williams K. Mobile elements bounded by C4A4 telomeric repeats in Oxytricha fallax. Cell. 1985 Dec;43(3 Pt 2):759–768. doi: 10.1016/0092-8674(85)90249-1. [DOI] [PubMed] [Google Scholar]
- Hunter D. J., Williams K., Cartinhour S., Herrick G. Precise excision of telomere-bearing transposons during Oxytricha fallax macronuclear development. Genes Dev. 1989 Dec;3(12B):2101–2112. doi: 10.1101/gad.3.12b.2101. [DOI] [PubMed] [Google Scholar]
- Jahn C. L., Krikau M. F., Shyman S. Developmentally coordinated en masse excision of a highly repetitive element in E. crassus. Cell. 1989 Dec 22;59(6):1009–1018. doi: 10.1016/0092-8674(89)90757-5. [DOI] [PubMed] [Google Scholar]
- Jahn C. L., Nilles L. A., Krikau M. F. Organization of the Euplotes crassus micronuclear genome. J Protozool. 1988 Nov;35(4):590–601. doi: 10.1111/j.1550-7408.1988.tb04157.x. [DOI] [PubMed] [Google Scholar]
- Jahn C. L., Prescott K. E., Waggener M. W. Organization of the micronuclear genome of oxytricha nova. Genetics. 1988 Sep;120(1):123–134. doi: 10.1093/genetics/120.1.123. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jahn C. L. The nuclear genomes of hypotrichous ciliates: maintaining the maximum and the minimum of information. J Protozool. 1991 May-Jun;38(3):252–258. doi: 10.1111/j.1550-7408.1991.tb04438.x. [DOI] [PubMed] [Google Scholar]
- Klobutcher L. A., Jahn C. L. Developmentally controlled genomic rearrangements in ciliated protozoa. Curr Opin Genet Dev. 1991 Oct;1(3):397–403. doi: 10.1016/s0959-437x(05)80306-5. [DOI] [PubMed] [Google Scholar]
- Klobutcher L. A. Micronuclear organization of macronuclear genes in the hypotrichous ciliate Oxytricha nova. J Protozool. 1987 Nov;34(4):424–428. doi: 10.1111/j.1550-7408.1987.tb03206.x. [DOI] [PubMed] [Google Scholar]
- Kraut H., Lipps H. J., Prescott D. M. The genome of hypotrichous ciliates. Int Rev Cytol. 1986;99:1–28. doi: 10.1016/s0074-7696(08)61422-9. [DOI] [PubMed] [Google Scholar]
- Krikau M. F., Jahn C. L. Tec2, a second transposon-like element demonstrating developmentally programmed excision in Euplotes crassus. Mol Cell Biol. 1991 Sep;11(9):4751–4759. doi: 10.1128/mcb.11.9.4751. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mitcham J. L., Lynn A. J., Prescott D. M. Analysis of a scrambled gene: the gene encoding alpha-telomere-binding protein in Oxytricha nova. Genes Dev. 1992 May;6(5):788–800. doi: 10.1101/gad.6.5.788. [DOI] [PubMed] [Google Scholar]
- Prescott D. M., Murti K. G., Bostock C. J. Genetic apparatus of Stylonychia sp. Nature. 1973 Apr 27;242(5400):576, 597-600. doi: 10.1038/242576a0. [DOI] [PubMed] [Google Scholar]
- Saiki R. K., Gelfand D. H., Stoffel S., Scharf S. J., Higuchi R., Horn G. T., Mullis K. B., Erlich H. A. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science. 1988 Jan 29;239(4839):487–491. doi: 10.1126/science.2448875. [DOI] [PubMed] [Google Scholar]
- Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shippen-Lentz D., Blackburn E. H. Functional evidence for an RNA template in telomerase. Science. 1990 Feb 2;247(4942):546–552. doi: 10.1126/science.1689074. [DOI] [PubMed] [Google Scholar]
- Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
- Tausta S. L., Klobutcher L. A. Detection of circular forms of eliminated DNA during macronuclear development in E. crassus. Cell. 1989 Dec 22;59(6):1019–1026. doi: 10.1016/0092-8674(89)90758-7. [DOI] [PubMed] [Google Scholar]
- Tausta S. L., Turner L. R., Buckley L. K., Klobutcher L. A. High fidelity developmental excision of Tec1 transposons and internal eliminated sequences in Euplotes crassus. Nucleic Acids Res. 1991 Jun 25;19(12):3229–3236. doi: 10.1093/nar/19.12.3229. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wünning I. U., Lipps H. J. A transformation system for the hypotrichous ciliate Stylonychia mytilus. EMBO J. 1983;2(10):1753–1757. doi: 10.1002/j.1460-2075.1983.tb01653.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yu G. L., Blackburn E. H. Developmentally programmed healing of chromosomes by telomerase in Tetrahymena. Cell. 1991 Nov 15;67(4):823–832. doi: 10.1016/0092-8674(91)90077-c. [DOI] [PubMed] [Google Scholar]
- Zahler A. M., Prescott D. M. Telomere terminal transferase activity in the hypotrichous ciliate Oxytricha nova and a model for replication of the ends of linear DNA molecules. Nucleic Acids Res. 1988 Jul 25;16(14B):6953–6972. doi: 10.1093/nar/16.14.6953. [DOI] [PMC free article] [PubMed] [Google Scholar]