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. 1993 May 11;21(9):2273–2274. doi: 10.1093/nar/21.9.2273

A method to increase the cumulative cleavage efficiency of ribozymes: thermal cycling.

B Dropulić ¹, N H Lin ¹, K T Jeang ¹

PMCID: PMC309510 PMID: 8502581

Abstract

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

Beaudry A. A., Joyce G. F. Directed evolution of an RNA enzyme. Science. 1992 Jul 31;257(5070):635–641. doi: 10.1126/science.1496376. [DOI] [PubMed] [Google Scholar]
Berzal-Herranz A., Joseph S., Burke J. M. In vitro selection of active hairpin ribozymes by sequential RNA-catalyzed cleavage and ligation reactions. Genes Dev. 1992 Jan;6(1):129–134. doi: 10.1101/gad.6.1.129. [DOI] [PubMed] [Google Scholar]
Chen C. J., Banerjea A. C., Harmison G. G., Haglund K., Schubert M. Multitarget-ribozyme directed to cleave at up to nine highly conserved HIV-1 env RNA regions inhibits HIV-1 replication--potential effectiveness against most presently sequenced HIV-1 isolates. Nucleic Acids Res. 1992 Sep 11;20(17):4581–4589. doi: 10.1093/nar/20.17.4581. [DOI] [PMC free article] [PubMed] [Google Scholar]
Dropulić B., Lin N. H., Martin M. A., Jeang K. T. Functional characterization of a U5 ribozyme: intracellular suppression of human immunodeficiency virus type 1 expression. J Virol. 1992 Mar;66(3):1432–1441. doi: 10.1128/jvi.66.3.1432-1441.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
Haseloff J., Gerlach W. L. Simple RNA enzymes with new and highly specific endoribonuclease activities. Nature. 1988 Aug 18;334(6183):585–591. doi: 10.1038/334585a0. [DOI] [PubMed] [Google Scholar]
Lehman N., Joyce G. F. Evolution in vitro of an RNA enzyme with altered metal dependence. Nature. 1993 Jan 14;361(6408):182–185. doi: 10.1038/361182a0. [DOI] [PubMed] [Google Scholar]
Taylor N. R., Rossi J. J. Ribozyme-mediated cleavage of an HIV-1 gag RNA: the effects of nontargeted sequences and secondary structure on ribozyme cleavage activity. Antisense Res Dev. 1991 Summer;1(2):173–186. doi: 10.1089/ard.1991.1.173. [DOI] [PubMed] [Google Scholar]
Uhlenbeck O. C. A small catalytic oligoribonucleotide. Nature. 1987 Aug 13;328(6131):596–600. doi: 10.1038/328596a0. [DOI] [PubMed] [Google Scholar]
Xing Z., Whitton J. L. Ribozymes which cleave arenavirus RNAs: identification of susceptible target sites and inhibition by target site secondary structure. J Virol. 1992 Mar;66(3):1361–1369. doi: 10.1128/jvi.66.3.1361-1369.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]

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