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Coimmunoprecipitation of Vps10p-HA with clathrin is reduced in pep12Δ and vps34Δ but not in apl2Δ/mutants. Native immunoprecipitations were performed from ODY50 (WT/lane 1–3), ODY62 (apl2Δ/lane 4–6), ODY63 (pep12Δ/lane 7–9), and ODY54 (vps34Δ/lane 10–12) as described in Figure 1.
