Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1993 Jun 11;21(11):2649–2653. doi: 10.1093/nar/21.11.2649

A small RNA of Mycoplasma capricolum that resembles eukaryotic U6 small nuclear RNA.

C Ushida 1, A Muto 1
PMCID: PMC309594  PMID: 7687343

Abstract

Mycoplasma capricolum, a parasitic prokaryote, contains several small stable RNAs, besides rRNAs and tRNAs. One of them, designated MCS4 RNA (125 nucleotides in length), has been isolated and sequenced. This RNA is abundant in the cell, and is encoded by two genes. Unexpectedly, MCS4 RNA has been found to reveal extensive sequence similarity to eukaryotic U6 snRNAs. This finding suggests that MCS4 and U6 snRNAs are derived from a common ancestral RNA that has existed before the divergence of prokaryotes and eukaryotes.

Full text

PDF
2649

Images in this article

2650Image
on p.2650
2650Image
on p.2650
2651Image
on p.2651

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andachi Y., Yamao F., Muto A., Osawa S. Codon recognition patterns as deduced from sequences of the complete set of transfer RNA species in Mycoplasma capricolum. Resemblance to mitochondria. J Mol Biol. 1989 Sep 5;209(1):37–54. doi: 10.1016/0022-2836(89)90168-x. [DOI] [PubMed] [Google Scholar]
  2. Bothwell A. L., Garber R. L., Altman S. Nucleotide sequence and in vitro processing of a precursor molecule to Escherichia coli 4.5 S RNA. J Biol Chem. 1976 Dec 10;251(23):7709–7716. [PubMed] [Google Scholar]
  3. Bourgaize D. B., Fournier M. J. Initiation of translation is impaired in E. coli cells deficient in 4.5S RNA. Nature. 1987 Jan 15;325(6101):281–284. doi: 10.1038/325281a0. [DOI] [PubMed] [Google Scholar]
  4. Brow D. A., Guthrie C. Spliceosomal RNA U6 is remarkably conserved from yeast to mammals. Nature. 1988 Jul 21;334(6179):213–218. doi: 10.1038/334213a0. [DOI] [PubMed] [Google Scholar]
  5. Brownlee G. G. Sequence of 6S RNA of E. coli. Nat New Biol. 1971 Feb 3;229(5):147–149. doi: 10.1038/newbio229147a0. [DOI] [PubMed] [Google Scholar]
  6. Busch H., Reddy R., Rothblum L., Choi Y. C. SnRNAs, SnRNPs, and RNA processing. Annu Rev Biochem. 1982;51:617–654. doi: 10.1146/annurev.bi.51.070182.003153. [DOI] [PubMed] [Google Scholar]
  7. Chauhan A. K., Apirion D. The gene for a small stable RNA (10Sa RNA) of Escherichia coli. Mol Microbiol. 1989 Nov;3(11):1481–1485. doi: 10.1111/j.1365-2958.1989.tb00133.x. [DOI] [PubMed] [Google Scholar]
  8. Das G., Henning D., Reddy R. Structure, organization, and transcription of Drosophila U6 small nuclear RNA genes. J Biol Chem. 1987 Jan 25;262(3):1187–1193. [PubMed] [Google Scholar]
  9. Donis-Keller H. Phy M: an RNase activity specific for U and A residues useful in RNA sequence analysis. Nucleic Acids Res. 1980 Jul 25;8(14):3133–3142. doi: 10.1093/nar/8.14.3133. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Epstein P., Reddy R., Henning D., Busch H. The nucleotide sequence of nuclear U6 (4.7 S) RNA. J Biol Chem. 1980 Sep 25;255(18):8901–8906. [PubMed] [Google Scholar]
  11. Guerrier-Takada C., Gardiner K., Marsh T., Pace N., Altman S. The RNA moiety of ribonuclease P is the catalytic subunit of the enzyme. Cell. 1983 Dec;35(3 Pt 2):849–857. doi: 10.1016/0092-8674(83)90117-4. [DOI] [PubMed] [Google Scholar]
  12. Guthrie C., Patterson B. Spliceosomal snRNAs. Annu Rev Genet. 1988;22:387–419. doi: 10.1146/annurev.ge.22.120188.002131. [DOI] [PubMed] [Google Scholar]
  13. Harada F., Kato N., Nishimura S. The nucleotide sequence of nuclear 4.8S RNA of mouse cells. Biochem Biophys Res Commun. 1980 Aug 14;95(3):1332–1340. doi: 10.1016/0006-291x(80)91620-4. [DOI] [PubMed] [Google Scholar]
  14. Hori H., Sawada M., Osawa S., Murao K., Ishikura H. The nucleotide sequence of 5S rRNA from Mycoplasma capricolum. Nucleic Acids Res. 1981 Oct 24;9(20):5407–5410. doi: 10.1093/nar/9.20.5407. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Inouye M., Delihas N. Small RNAs in the prokaryotes: a growing list of diverse roles. Cell. 1988 Apr 8;53(1):5–7. doi: 10.1016/0092-8674(88)90480-1. [DOI] [PubMed] [Google Scholar]
  16. Kawauchi Y., Muto A., Osawa S. The protein composition of Mycoplasma capricolum. Mol Gen Genet. 1982;188(1):7–11. doi: 10.1007/BF00332989. [DOI] [PubMed] [Google Scholar]
  17. Kiss T., Antal M., Solymosy F. Plant small nuclear RNAs. II. U6 RNA and a 4.5SI-like RNA are present in plant nuclei. Nucleic Acids Res. 1987 Jan 26;15(2):543–560. doi: 10.1093/nar/15.2.543. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Komine Y., Inokuchi H. Physical map locations of the genes that encode small stable RNAs in Escherichia coli. J Bacteriol. 1991 Sep;173(17):5252–5252. doi: 10.1128/jb.173.17.5252.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Kunkel G. R., Maser R. L., Calvet J. P., Pederson T. U6 small nuclear RNA is transcribed by RNA polymerase III. Proc Natl Acad Sci U S A. 1986 Nov;83(22):8575–8579. doi: 10.1073/pnas.83.22.8575. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Muto A., Andachi Y., Yuzawa H., Yamao F., Osawa S. The organization and evolution of transfer RNA genes in Mycoplasma capricolum. Nucleic Acids Res. 1990 Sep 11;18(17):5037–5043. doi: 10.1093/nar/18.17.5037. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Muto A., Yamao F., Osawa S. The genome of Mycoplasma capricolum. Prog Nucleic Acid Res Mol Biol. 1987;34:29–58. doi: 10.1016/s0079-6603(08)60492-4. [DOI] [PubMed] [Google Scholar]
  22. Orum H., Nielsen H., Engberg J. Spliceosomal small nuclear RNAs of Tetrahymena thermophila and some possible snRNA-snRNA base-pairing interactions. J Mol Biol. 1991 Nov 20;222(2):219–232. doi: 10.1016/0022-2836(91)90208-n. [DOI] [PubMed] [Google Scholar]
  23. Poritz M. A., Bernstein H. D., Strub K., Zopf D., Wilhelm H., Walter P. An E. coli ribonucleoprotein containing 4.5S RNA resembles mammalian signal recognition particle. Science. 1990 Nov 23;250(4984):1111–1117. doi: 10.1126/science.1701272. [DOI] [PubMed] [Google Scholar]
  24. Razin S. Molecular biology and genetics of mycoplasmas (Mollicutes). Microbiol Rev. 1985 Dec;49(4):419–455. doi: 10.1128/mr.49.4.419-455.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Reed R. E., Baer M. F., Guerrier-Takada C., Donis-Keller H., Altman S. Nucleotide sequence of the gene encoding the RNA subunit (M1 RNA) of ribonuclease P from Escherichia coli. Cell. 1982 Sep;30(2):627–636. doi: 10.1016/0092-8674(82)90259-8. [DOI] [PubMed] [Google Scholar]
  26. Samuelsson T., Guindy Y. Nucleotide sequence of a Mycoplasma mycoides RNA which is homologous to E. coli 4.5S RNA. Nucleic Acids Res. 1990 Aug 25;18(16):4938–4938. doi: 10.1093/nar/18.16.4938. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Sawa H., Shimura Y. Association of U6 snRNA with the 5'-splice site region of pre-mRNA in the spliceosome. Genes Dev. 1992 Feb;6(2):244–254. doi: 10.1101/gad.6.2.244. [DOI] [PubMed] [Google Scholar]
  28. Sawada M., Muto A., Iwami M., Yamao F., Osawa S. Organization of ribosomal RNA genes in Mycoplasma capricolum. Mol Gen Genet. 1984;196(2):311–316. doi: 10.1007/BF00328064. [DOI] [PubMed] [Google Scholar]
  29. Shumyatsky G., Reddy R. Compilation of small RNA sequences. Nucleic Acids Res. 1992 May 11;20 (Suppl):2159–2165. doi: 10.1093/nar/20.suppl.2159. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Simoneau P., Hu P. C. The gene for a 4.5S RNA homolog from Mycoplasma pneumoniae: genetic selection, sequence, and transcription analysis. J Bacteriol. 1992 Jan;174(2):627–629. doi: 10.1128/jb.174.2.627-629.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Skinner H. B., Adams D. S. Nucleotide sequence of Physarum U6 small RNA. Nucleic Acids Res. 1987 Jan 12;15(1):371–371. doi: 10.1093/nar/15.1.371. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Tanaka R., Andachi Y., Muto A. Evolution of tRNAs and tRNA genes in Acholeplasma laidlawii. Nucleic Acids Res. 1991 Dec 25;19(24):6787–6792. doi: 10.1093/nar/19.24.6787. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Tani T., Ohshima Y. The gene for the U6 small nuclear RNA in fission yeast has an intron. Nature. 1989 Jan 5;337(6202):87–90. doi: 10.1038/337087a0. [DOI] [PubMed] [Google Scholar]
  34. Tani T., Ohshima Y. mRNA-type introns in U6 small nuclear RNA genes: implications for the catalysis in pre-mRNA splicing. Genes Dev. 1991 Jun;5(6):1022–1031. doi: 10.1101/gad.5.6.1022. [DOI] [PubMed] [Google Scholar]
  35. Tschudi C., Krainer A. R., Ullu E. The U6 small nuclear RNA from Trypanosoma brucei. Nucleic Acids Res. 1988 Dec 9;16(23):11375–11375. doi: 10.1093/nar/16.23.11375. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Walker R. T., RajBhandary U. L. The nucleotide sequence of formylmethionine tRNA from Mycoplasma mycoides sp. capri. Nucleic Acids Res. 1978 Jan;5(1):57–70. doi: 10.1093/nar/5.1.57. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Whitley J. C., Muto A., Finch L. R. A physical map for Mycoplasma capricolum Cal. kid with loci for all known tRNA species. Nucleic Acids Res. 1991 Jan 25;19(2):399–400. doi: 10.1093/nar/19.2.399. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Zucker-Aprison E., Thomas J. D., Blumenthal T. C. elegans snRNAs: a model for U4/U6 base pairing. Nucleic Acids Res. 1988 Jul 25;16(14B):7188–7188. doi: 10.1093/nar/16.14.7188. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES