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. 1993 Jun 11;21(11):2655–2661. doi: 10.1093/nar/21.11.2655

Selective binding of the estrogen receptor to one strand of the estrogen responsive element.

R Mukherjee 1
PMCID: PMC309595  PMID: 8332462

Abstract

The human estrogen receptor (hER) activates gene transcription by binding to cognate palindromic sequences called estrogen responsive elements (ERE). I used gel retardation assays and oligonucleotides containing the ERE from the Xenopus vitellogenin gene to study the interaction of the hER with the ERE. I observed that the hER bound to double-stranded ERE and to the single strand of the ERE that had T in the center with nearly equal affinity, but not to the strand which had A in the center. Interchanging the two central nucleotides changed the strand specificity. Binding of the hER to a single strand is extremely sensitive to temperature. Initial recognition of one of the two strands of the ERE may be involved in the binding of the hER to the ERE.

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