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. 1993 Aug 11;21(16):3917–3918. doi: 10.1093/nar/21.16.3917

A novel method employing UNG to avoid carry-over contamination in RNA-PCR.

Udaykumar 1, J S Epstein 1, I K Hewlett 1
PMCID: PMC309940  PMID: 8367319

Abstract

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987 Apr;162(1):156–159. doi: 10.1006/abio.1987.9999. [DOI] [PubMed] [Google Scholar]
  2. Kwok S., Higuchi R. Avoiding false positives with PCR. Nature. 1989 May 18;339(6221):237–238. doi: 10.1038/339237a0. [DOI] [PubMed] [Google Scholar]
  3. Thornton C. G., Hartley J. L., Rashtchian A. Utilizing uracil DNA glycosylase to control carryover contamination in PCR: characterization of residual UDG activity following thermal cycling. Biotechniques. 1992 Aug;13(2):180–184. [PubMed] [Google Scholar]

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